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nCounter Analysis System

MANUFACTURER NanoString Technologies(manufacturer's website)
MODEL nCounter

Division of Biosciences

RESTRICTIONS Training only for projects larger than 48 samples
TRAINING Training is required to use this item and we can arrange this if needed.
CONTACT 1 Paola Oliveri
CONTACT 2 Fraser Simpson
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SITE Bloomsbury Campus

Description

An automated, multi-application, digital detection and counting system which directly profiles up to 800 molecules simultaneously.

The nCounter system is fully automated and uses fluorescently labelled reporter probes, called 'codesets', and single molecule imaging to detect and count hundreds of molecules in a single reaction. The system is very resistant to poor quality of the RNA samples and it is perfectly suited for RNA extracted from FFPE (formalin-fixed, paraffin-embedded) samples.

The nCounter technology has sensitivity and specificity comparable to real time PCR (RT-PCR) and it is faster than NGS. This technology takes advantage of the well-understood nucleic acid hybridization reaction and no enzymatic reactions are involved, no reverse transcription or DNA amplification is necessary.

Simultaneous quantification of combinations of DNA, RNA, microRNA and protein targets up to 800-plex from a single sample.

Specification

The System is is made of a Prep Station (liquid handling robot) and a Digital analyzer both working at room temperature. Each run is for 12 samples.

Upgrades

Digital Analyzer hardware upgrade in March 2019

Future Upgrades

Item ID #729.

Last Updated: 30th October, 2019

nCounter Analysis System

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