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|Institute of Child Health
The MoFlo XDP is a high-speed cell sorter.
MoFlo XDP is equipped with 3 lasers: UV 355nm (100mW), blue 488 nm (200mW), and red 647 nm (100mW). It can detect 2 scatters and 9 fluorescence parameters (5 colours on the blue laser, 2 colours on the UV laser, and 2 colours on the red diode) with digital pulse processing at a resolution of 32-bit and 5-decade multi-channel digital dynamic range. The MoFlo XDP, like the old MoFlo Legacy, has 4-way sort capability and water-bath controlled temperature of both pre- and post-sorted cells. The IntelliSort software, which replaces the earlier SortMaster, monitors temperature and pressure and controls stream stability. The MoFlo XDP is controlled via a touch screen and can sort cells at speeds of up to 70,000 cells per second. CyClone single cell cloning is very fast(single cell cloning in a 96-well plate takes less than a minute), easy to set up and allows the customisation of many plate configurations. The MoFlo XDP can detect most standard flow cytometry fluorochromes plus GFP, YFP, RFP, and UV excited DNA dyes such as Hoechst 33342 and DAPI. APC, APC-Cy7 and APC-H7 are also detected with reasonably good sensitivity.
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Last Updated: 10th November, 2015