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|CONTACT 1||Jonathan Knowles|
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|SITE||Eastman Dental Institute|
With both Raman and FTIR spectroscopy peaks are obtained at high wavenumbers due to known specific chemical groups. In the low wavenumber fingerprint region the spectra are generally unique for a given chemical. Raman and FTIR are considered to provide complimentary spectra because peaks for a specific chemical group of interest that may be weak in one spectrum is likely to be strong in the other. These techniques have been available to study the bulk properties of chemicals for many years but with the advent of Raman and FTIR microsocopy it is now possible to gain a chemical map of a surface with micron resolution.
The Labram 300 (Horiba Jobin Yvon) is equipped with a HeNe (633nm) laser and an xyz mapping stage. With the 1800 and 950 Raman gratings wavenumber resolutions of 1 and 2cm-1 respectively are obtained. Use of a video camera allows white light images of a sample on the microscope to be transferred to the computer screen. The data acquisition software then enables spectra to be obtained within selected areas in the image. Data can also be obtained as a function of time and depth. With the analysis options the sample spectra can be compared with those of known compounds. Coloured images, providing information on the relative amounts of each chemical at different points within the region mapped, are then generated.
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Last Updated: 16th July, 2014